Volume 3, Issue 1 (1-2018)                   hrjbaq 2018, 3(1): 29-35 | Back to browse issues page

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Masoumalinejad Z, Zinatizadeh M R. Molecular Identification of Human Papilloma Virus in Tissue Samples from Patients with Cervical Cancer by Multiplex PCR Method. hrjbaq. 2018; 3 (1) :29-35
URL: http://hrjbaq.ir/article-1-211-en.html
Department of Genetics, Faculty of Science, Tonekabon Branch of Islamic Azad University, Mazandaran, Iran , (zinati3333@gmail.com)
Abstract:   (1884 Views)

Introduction: Contamination with high-risk human papillomavirus (HPV) is one of the most important risk factors for developing cervix cancer. Since there is no possibility of detecting the virus and its subtypes by using current biochemical and serological methods and cell culture, the molecular methods such as multiplex PCR have particular importance in accurate, early and definite diagnosis of this virus. So, in this research, our goal is to use a proprietary multiplex PCR assay based on HPV-16 and HPV-18 of human papillomavirus for molecular recognition of HPV and to evaluate its prevalence in cervix cancer patients.
Materials and Methods: In this experimental study, after collecting samples from malignant cervical lesions of the 60 patients, the viral DNA was extracted and multiplex PCR was done by specific primers of human papillomavirus in all samples. After the analysis of multiplex PCR products by 1% agarose gel electrophoresis, specificity of the test was also evaluated.
Results: Among 60 samples, 19 cases were confirmed to be positive for HPV contamination and 41 cases were negative. So the prevalence of HPV contamination was reported 30% in this population.
Conclusion: This study showed that Multiplex PCR by specific primers for HPV-16 and HPV-18 of human papilloma virus is a proper and accurate method for detection of this virus and the results also confirm the previous reports of correlation between HPV and cervical carcinoma in iranian population.

Full-Text [PDF 562 kb]   (486 Downloads)    
Type of Study: Research | Subject: Special
Received: 2017/12/7 | Revised: 2018/11/7 | Accepted: 2018/01/27 | ePublished ahead of print: 2018/01/28 | Published: 2018/01/28

1. Parkin DM. Global cancer statistics in the year 2000. Lancet Oncol. 2001;2(9):533-43. doi: 10.1016/S1470-2045(01)00486-7 pmid: 11905707
2. Longnecker MP, Bernstein L, Paganini-Hill A, Enger SM, Ross RK. Risk factors for in situ breast cancer. Cancer Epidemiol Biomarkers Prev. 1996;5(12):961-5. pmid: 8959317
3. Peedicayil A, Abraham P, Sathish N, John S, Shah K, Sridharan G, et al. Human papillomavirus genotypes associated with cervical neoplasia in India. Int J Gynecol Cancer. 2006;16(4):1591-5. doi: 10.1111/j.1525-1438.2006.00631.x pmid: 16884371
4. Burd EM. Human papillomavirus and cervical cancer. Clin Microbiol Rev. 2003;16(1):1-17. pmid: 12525422
5. Franco EL, Duarte-Franco E, Ferenczy A. Cervical cancer: epidemiology, prevention and the role of human papillomavirus infection. CMAJ. 2001;164(7):1017-25. pmid: 11314432
6. Baliga MS, Dsouza JJ. Amla (Emblica officinalis Gaertn), a wonder berry in the treatment and prevention of cancer. Eur J Cancer Prev. 2011;20(3):225-39. doi: 10.1097/CEJ.0b013e32834473f4 pmid: 21317655
7. Czegledy J, Gergely L, Hernadi Z, Poka R. Detection of human papillomavirus deoxyribonucleic acid in the female genital tract. Med Microbiol Immunol. 1989;178(6):309-14. pmid: 2559306
8. Malloy C, Sherris J, Herdman C. HPV DNA Testing: Technical and Programmatic Issues for Cervical Cancer Prevention in Low-Resource Settings. 2000.
9. Ritter DB, Kadish AS, Vermund SH, Romney SL, Villari D, Burk RD. Detection of human papillomavirus deoxyribonucleic acid in exfoliated cervicovaginal cells as a predictor of cervical neoplasia in a high-risk population. Am J Obstet Gynecol. 1988;159(6):1517-25. pmid: 2849881
10. Singh A, Datta P, Jain SK, Bhatla N, Dutta Gupta S, Dey B, et al. Human papilloma virus genotyping, variants and viral load in tumors, squamous intraepithelial lesions, and controls in a north Indian population subset. Int J Gynecol Cancer. 2009;19(9):1642-8. doi: 10.1111/IGC.0b013e3181a83555 pmid: 19955952
11. Shukla S, Bharti AC, Mahata S, Hussain S, Kumar R, Hedau S, et al. Infection of human papillomaviruses in cancers of different human organ sites. Indian J Med Res. 2009;130(3):222-33. pmid: 19901431
12. Lazo PA. The molecular genetics of cervical carcinoma. Br J Cancer. 1999;80(12):2008-18. doi: 10.1038/sj. bjc.6690635 pmid: 10471054
13. Zanotti KM, Kennedy AW. Screening for gynecologic cancer. Med Clin North Am. 1999;83(6):1467-87. pmid: 10584603
14. Crosbie EJ, Einstein MH, Franceschi S, Kitchener HC. Human papillomavirus and cervical cancer. Lancet. 2013;382(9895):889-99. doi: 10.1016/S0140-6736(13)60022-7 pmid: 23618600
15. Konidaris S, Kouskouni EE, Panoskaltsis T, Kreatsas G, Patsouris ES, Sarivalassis A, et al. Human papillomavirus infection in malignant and benign gynaecological conditions: a study in Greek women. Health Care Women Int. 2007;28(2):182-91. doi: 10.1080/0739933 0601128627 pmid: 17364979
16. Schiffman MH, Castle P. Epidemiologic studies of a necessary causal risk factor: human papillomavirus infection and cervical neoplasia. J Natl Cancer Inst. 2003;95(6):E2. pmid: 12644550
17. Li S, Meng YH, Ting H, Shen J, Ma D. Clinical significance of human papilloma virus infection in the cervical lesions. Front Med China. 2010;4(3):264-70. doi: 10.1007/s11684-010-0094-6 pmid: 21191829
18. Bazuaye PE. Cervical dysplasia in Jamaica women: lifestyle and genetic factors: The University of the West Indies; 2012.
19. Castle PE, Giuliano AR. Chapter 4: Genital tract infections, cervical inflammation, and antioxidant nutrients--assessing their roles as human papillomavirus cofactors. J Natl Cancer Inst Monogr. 2003(31):29-34. pmid: 12807942
20. Mostafavizadeh SM, Niakan M, Ahmadi A, Aghabozorgi S, Lak R, Piroozmand A. Frequency distribution of HPV18 based on the detection of E6 oncoprotein gene in cervix cancer samples. Feyz J Kashan Univ Med Sci. 2013;17(3).
21. Bosch FX, Manos MM, Munoz N, Sherman M, Jansen AM, Peto J, et al. Prevalence of human papillomavirus in cervical cancer: a worldwide perspective. International biological study on cervical cancer (IBSCC) Study Group. J Natl Cancer Inst. 1995;87(11):796-802. pmid: 7791229
22. Sifuentes Alvarez A, Reyes Romero M. [Risk factors for cervico-uterine cancer associated to HPV: p53 codon 72 polymorphism in women attending hospital care]. Ginecol Obstet Mex. 2003;71:12-5. pmid: 12708345
23. Hamkar R, Azad TM, Mahmoodi M, Seyedirashti S, Severini A, Nategh R. Prevalence of human papillomavirus in Mazandaran Province, Islamic Republic of Iran. East Mediterr Health J. 2002;8(6):805-11. pmid: 15568458
24. Nasiri S, Ghalamkarpoor F, Saberi A, Vesal P. [Study of human papilloma virus in anogenital condylomas by PCR method]. Arch Clin Infect Dis. 2008;3(1).
25. Park JS, Namkoong SE, Lee JM, Kim EJ, Chee YH, Han GT, et al. Cervical intraepithelial neoplasia 3, coinfected with HPV-16 and -18--case report. J Korean Med Sci. 1993;8(2):162-5. doi: 10.3346/jkms.1993.8.2.162 pmid: 8397933
26. Johnson R. Viral infections of the nervous system: Lippincott-Raven Publishers; 1998.
27. Schiller JT, Hidesheim A. Developing HPV virus-like particle vaccines to prevent cervical cancer: a progress report. J Clin Virol. 2000;19(1-2):67-74. pmid: 11091149
28. Meshkat Z, Soleimanjahi H, Mahmoudi M, Mirshahabi H, Hassan ZM, Ghaffari SR, et al. Determination of human papillomavirus type 16 genotype and construction of cloning vector pTZ57R encoding HPV16 E7 gene. Saudi Med J. 2007;28(10):1511-5. pmid: 17914510
29. Stamenkovic M, Knezevic A, Knezevic I, Kuzmanovic I, Karalic D, Milenkovic S, et al. High-risk human papilloma virus genotypes in cervical carcinoma of Serbian women: Distribution and association with pathohistological findings. Biologicals. 2016;44(5):412-6. doi: 10.1016/j.biologicals.2016.05.001 pmid: 27461 126
30. Keyhani E, Kohannia N, Izadimood N, Keyhkhaee M, Najmabadi H. The prevalence of human papilloma virus (HPV) in malignant cervical lesion, using multiplex PCR. Tehran Univ Med J TUMS Publ. 2006;64(3):95-101.
31. Jabbar Poor M. Determination of various types of human papillomavirus enucleation in cervical cancer lesions in north west of Iran. Q Tropic Dis J. 2007;13(41):29-34.
32. Kan CY, Iacopetta BJ, Lawson JS, Whitaker NJ. Identification of human papillomavirus DNA gene sequences in human breast cancer. Br J Cancer. 2005;93(8):946-8. doi: 10.1038/sj.bjc.6602778 pmid: 16222323
33. Vidal AC, Murphy SK, Hernandez BY, Vasquez B, Bartlett JA, Oneko O, et al. Distribution of HPV genotypes in cervical intraepithelial lesions and cervical cancer in Tanzanian women. Infect Agent Cancer. 2011;6(1):20. doi: 10.1186/1750-9378-6-20 pmid: 220 81870
34. Xu QX, Zhang ZY. High-risk human papillomavirus genotypes in cervical lesions and vaccination challenges in China. Asian Pac J Cancer Prev. 2015;16(6):2193-7. pmid: 25824736
35. Niakan M. [Human papillomavirus diagnosis in cervical cancer lesions by molecular hybridization]. Q J Fertil Infertil. 1998;8(2):18-22.
36. McNair R, Power J, Carr S. Comparing knowledge and perceived risk related to the human papilloma virus among Australian women of diverse sexual orientations. Aust N Z J Public Health. 2009;33(1):87-93. doi: 10.1111/j.1753-6405.2009.00345.x pmid: 19236366

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