Volume 7, Issue 4 (9-2022)                   hrjbaq 2022, 7(4): 300-313 | Back to browse issues page

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Asmar A, Azarbayjani M A, Peeri M. The Effect of Endurance Training and Octopamine on the Expression of Proteins and Genes Related to Apoptosis in the Cerebellum of Male Rats Fed with Deep-Fried oil. hrjbaq 2022; 7 (4) :300-313
URL: http://hrjbaq.ir/article-1-558-en.html
Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran , (m_azarbayjani@iauctb.ac.ir)
Abstract:   (456 Views)

Introduction: Cooking food with deep-fried oils (DFO) can cause neurodegenerative damage due to the presence of toxic compounds. Considering the increasing consumption of ready-to-cook foods, this study aimed to determine the effect of endurance training and octopamine on apoptotic genes and proteins and number of Purkinje cells in cerebellum of rats fed with DFO.
Materials and Methods: 30 male Wistar rats (300-350 g) were randomly divided into 5 groups as control, control receiving DFO, DFO-endurance training, DFO-octopamine, and DFO-endurance training-octopamine. After the rats were treated with DFO through gavage for five days a week (1 cc/kg), dissolved octopamine was administered (81 μmol/kg/ip/4 weeks). Endurance training was performed on a treadmill at a speed of 26 meters per minute, 20 minutes per day and 5 sessions per week. After 4 weeks, the rats were sacrificed and the cerebellum tissue was separated. Then the expression of the Pro-Caspase-3 protein was detected using the Western blot method, and the caspase-3, BAX, Bcl-2, and BAX genes were expressed using the Real Time PCR method.
Results: The expression of Bcl-2 gene and the number of cerebellar Purkinje cells in rats receiving DFO significantly decreased. But, the expression of Bax, Bax/Bcl-2, Caspase-3, Caspase-8, Pro Caspase-3 protein and percentage of apoptotic cells increased significantly (p<0.05). Exercise caused a significant increase in the expression of Bcl-2 gene and a significant decrease in the expression of Bax, Bax/Bcl-2, Caspase-3, Caspase-8, Pro Caspase-3 protein and the percentage of apoptotic cells (P<0.05). octopamine therapy led to a significant increase in the expression of Bcl-2 gene and a significant decrease in the expression of Bax, Bax/Bcl-2, Caspase-3, Caspase-8, Pro-Caspase-3 protein and the percentage of apoptotic cells (P<0.05). However, other interactions were not significant.
Conclusion: Eating deep-fried foods cause oxidative damage in the cerebellar tissue, and it seems that endurance exercise and octopamine consumption can ameliorate these injuries by reducing apoptotic events.

Author Contribution: All authors contributed equally in this work.
Conflict of Interest/Funding/Supports: None
Ethical Considerations: All ethical issues were considered in this study. Also, it was approved in ethical committee for Working with Laboratory Animals of Islamic Azad University (Ethical code: IR.IAU.K.REC.1398.086).
Applicable Remarks: To introduce the effects of endurance training and octopamine supplements to reduce the oxidative damage of the cerebellum tissue, which was impaired by DFO, and reduce the damage caused by DFO feeding.

 

Article number: 3
Full-Text [PDF 2000 kb]   (89 Downloads)    
Type of Study: Research | Subject: Special
Received: 2021/12/9 | Revised: 2022/11/14 | Accepted: 2022/05/16 | ePublished ahead of print: 2022/08/28 | Published: 2022/11/2

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